Awards/Fellowship/Recognition

• Technology certificate to develop CIRB Artificial Gun by ICAR (2024)
• Technology certificate to develop Digital AV for semen collection from bulls by ICAR (2024)
• Technology certificate to develop Spermoscope by ICAR (2024)
• Best presentation award by SAPI (2023)
• Limca Book of Records for ‘Most calves cloned from a single bull (2022)
• India Book of Record for ‘Maximum Buffalo Clones Produced (2021)
• Eulogy (प्रशस्ति पत्र) by Deputy Commissioner (DC) Hisar (2021)
• Team Award by ISBD- (2021)
• ICMR-DHR long-term international fellowships (2019)
• Appreciation certificate from Director CIRB for commercialization of technology (2020)
• Appreciation certificate from Director CIRB for outstanding performance and significant contribution to cloning (2020)
• Nanaji Deshmukh ICAR Award for Outstanding Interdisciplinary Team Research (2019)
• Nil Lagerlof Memorial Award by ISSAR (2019)
• Best poster award ISSAR (2018)
• Best Young Scientist Award ISSAR (2018)
• Best presentation award: (2016)

Membership of Professional Body

• Indian Society for the Study of Animal Reproduction (1755)
• Society for Physiologists of India (APA/265/2021)
• Indian Society for Buffalo Development (L0582)
• Society of Animal Physiology of India

Ongoing research project (PI)

Investigating molecular basis of seasonal variation on seminal attributes for identification of probable biomarkers of semen quality in buffaloes (DBT Funded).

Sequestrating X- and Y-sperm using receptor-ligand based approach in buffalo (Institute funded)

Publications

• Yadav PS, Kumar D, Saini M, Sharma RK, Dua S, Selokar NL, Bansal S, Punetha M, Gupta A, Kumar R, Kumar P. (2024). Evaluation of postnatal growth, hematology, telomere length and semen attributes of multiple clones and re-clone of superior buffalo breeding bulls. 2024 Jan 1;213:24-33.
• Kumar, D., Mehta, J.S., Andonissamy, J., Kumar, P., Kumar, D., Bhardwaj, S., Patil, C.S., Bala, R., Verma, N., Nain, S. and Sharma, R.K. (2024). Effect of season, age and period on semen quality traits in buffalo bulls. Acta Agriculturae Scandinavica, Section A—Animal Science, pp.1-7.
• Kumar, D., Mehta, J.S., Jerome, A., Kumar, P., Bhardwaj, S., Patil, C.S., Bala, R., Verma, N., Virmani, M., Sharma, R.K. and Singh, P. (2024). Cryopreservation alters buffalo sperm kinematics and mitochondrial parameters, acrosome and intra-cellular calcium status. Cryoletters, 45(4), pp.257-268.
• Punetha M, Saini Sl, Choudhary S, Sharma S, Bala R, Kumar P, Sharma RK, Yadav PS, Datta TK, Kumar D. (2024). Establishment of CRISPR-Cas9 ribonucleoprotein mediated MSTN gene edited pregnancy in buffalo: Compare cells transfection and zygotes electroporation. Theriogenology 229:158–168.
• Jinagal S, Dutt R, Sharma M, Punetha M, Saini S, Thakur S, Chaudhary S, Kumar P, Yadav PS, Datta TK, Kumar D. (2024). LPS-Induced Mitochondrial Dysfunction Reduces Oocyte Maturation and Developmental Competence of Buffalo Embryos via ROS Mediated TLR4 Signalling. Am J Reprod Immunol.; 92(1)
• Kumari, N., Saini, S., Thakur, S., Sharma, S., Punetha, M., Kumar, P., Sango, C., Sharma, R.K., Datta, T.K., Yadav, P.S. and Kumar, D. (2024). Enhancing the quality of inferior oocytes of buffalo for in vitro embryo production: The impact of melatonin on maturation, SCNT, and epigenetic modifications. Tissue and Cell, 89, p.102480.
• Sharma M, Punetha M, Saini S, Chaudhary S, Jinagal S, Thakur S, Kumar P, Kumar R, Sharma RK, Yadav PS, Kumar D. (2024). Mito-Q supplementation of in vitro maturation or in vitro culture medium improves maturation of buffalo oocytes and developmental competence of cloned embryos by reducing ROS production. Animal Reproduction Science ;260:107382.
• Yadav, H.P., Mohanty, T.K., Dewry, R.K., Lone, S.A., Nath, S., Bhakat, M., Baithalu, R.K., Tiwari, S., Swain, D.K., Kumar, P. and Mohanty, A.K. (2024). Reduced sperm number of murrah (Bubalus bubalis) bull semen in French mini-straw affects kinetic and functional competence after cryopreservation. Cryoletters, 45(1), pp.36-40.
• Kumar, A., Singh, G., Andonissamy, J., Kumar, P., Venkateshappa, A., Bala, R., Verma, N., Patil, C.S. and Sharma, R.K. (2023). Inter-relationship of peripheral hormones (IGF-1, testosterone and growth hormone) with reproductive traits in male buffalo. Buffalo Bulletin, 42(4), pp.467-477.
• Kumar, D., Mehta, J.S., Jerome, A., Kumar, P., Kumar, D., Shivani, B., Patil, C.S., Bala, R., Verma, N., Nain, S. and Sharma, R.K. (2023). Genetic analysis for semen quality traits in buffalo bulls. Tropical Animal Health and Production, 55(5), p.313.
• Punetha, M., Saini, S., Chaudhary, S., Bala, R., Sharma, M., Kumar, P., Kumar, D. and Yadav, P.S. (2023). Mitochondria-targeted antioxidant MitoQ ameliorates ROS production and improves cell viability in cryopreserved buffalo fibroblasts. Tissue and Cell, 82, p.102067.
• Dalal, Jasmer, Pradeep Kumar, R. K. Chandolia, Shikha Pawaria, Renu Bala, Dharmendra Kumar, and P. S. Yadav. (2023). A new role of H89: Reduces capacitation-like changes through inhibition of cholesterol efflux, calcium influx, and proteins tyrosine phosphorylation during sperm cryopreservation in buffalo.” Theriogenology204: 31-39.
• Yadav, U., Dutt, R., Bansal, K., Gupta, A., Bala, R., Bhardwaj, S., Verma, N., Bishnoi, M., Kumar, D., Datta, T.K. and Kumar, P. (2023). Epsilon poly‐lysine in buffalo semen extender: A step towards reducing the development of antibiotic resistance. Reproduction in Domestic Animals, 58(8), pp.1070-1079.
• Arjun, Venkateshappa, Pradeep Kumar, Ravi Dutt, Amit Kumar, Renu Bala, Nisha Verma, Andonissamy Jerome et al. (2022) Effect of mitochondria‐targeted antioxidant on the regulation of the mitochondrial function of sperm during cryopreservation. Andrologia: e14431.
• Diaz-Jimenez, Maria, Mengying Wang, Wanxue Wang, Evgenia Isachenko, Gohar Rahimi, Pradeep Kumar, Peter Mallmann, Melanie von Brandenstein, Manuel Hidalgo, and Vladimir Isachenko. (2022). Cryo-banking of human spermatozoa by aseptic cryoprotectants-free vitrification in liquid air: Positive effect of elevated warming temperature. Cell and Tissue Banking23, no. 1: 17-29.
• Pradeep Kumar, Mengying Wang, Evgenia Isachenko, Gohar Rahimi, Peter Mallmann, Wanxue Wang, Melanie von Brandenstein and Vladimir Isachenko. (2021). Unravelling subcellular and ultrastructural changes during vitrification of human spermatozoa: effect of mitochondria- targeted antioxidant and permeable Frontiers in Cell and Developmental Biology, 9, 1515
• Wang, M., Todorov, P., Isachenko, E., Rahimi, G., Wang, W., von Brandenstein, M., Kumar, Pradeep., Mallmann, and Isachenko, V. (2021). Aseptic capillary vitrification of human spermatozoa: cryoprotectant-free vs. cryoprotectant-included technologies. Cryobiology. 99, 95- 102.
• Kumar, A., Singh, G., Jerome, A., Kumar, P., Arjun, V., Bala, R., Verma, N. and Sharma, R.K. (2021).  IGF-1 supplementation in semen affects mitochondrial functional and calcium status of buffalo sperm following cryopreservation. Animal Reproduction Science, 231, 106783
• Lone, S.A., Mohanty, T.K., Bhakat, M., Yadav, H.P., Paray, A.R., Dewry, R.K., Baithalu, R.K., Sinha, R. and Kumar, P., (2021). Cholesterol‐loaded cyclodextrin attenuates dilution effect and improves    quality    of    bovine    low    sperm    insemination    doses    during Andrologia, 53(10), p.e14202.
• Dalal, J., Chandolia, R.K., Pawaria, S., Kumar, A., Kumar, D., Selokar, N.L., Andonissamy, J., Yadav, P.S. and Kumar, P. (2020). Low‐density lipoproteins protect sperm during cryopreservation in buffalo: Unraveling mechanism of Molecular Reproduction and Development.
• Dalal, J., Chandolia, R.K., Jan, M.H., Pawaria, S., Verma, N., Jerome, A., Kumar, D. and Kumar, P. (2020). Escherichia coli membrane‐derived oxygen‐reducing enzyme system (Oxyrase) protects bubaline spermatozoa during cryopreservation. Molecular Reproduction and Development,87(10), pp.1048-1058.
• Bhardwaj, S., Kumar, P., Jerome, A., Ravesh, S., Patil, C., Singh, P. and Lailer, P.C. (2020). Serum kisspeptin: New possible biomarker for sexual behaviour and sperm concentration in buffalo bulls. Reproduction in Domestic Animals, 55(9), pp.1190-1201.
• Patil, S., Kumar, P., Singh, G., Bala, R., Jerome, A., Patil, C. S., … & Sharma, K. (2020). ‘Semen dilution effect’on sperm variables and conception rate in buffalo. Animal Reproduction Science, 214, 106304.
• Lone, S. A., Mohanty, T. K., Bhakat, M., Paray, A. R., Yadav, H. P., Singh, A., … & Kumar, P. (2020). Modification of French mini-straw plug position for cryopreservation of small doses of bull Animal Reproduction Science, 218, 106485.
• Dalal, J., Kumar, P., Chandolia, R. K., Pawaria, S., Rajendran, R., Sheoran, S., … & Kumar, D. (2019). A new role for RU486 (mifepristone): it protects sperm from premature capacitation during cryopreservation in Scientific Reports, 9(1), 1-10.
• Selokar, N.L., Sharma, P., Saini, M., Sheoran, S., Rajendran, R., Kumar, D., Sharma, R.K., Motiani, R.K., Kumar, P., Jerome, A. and Khanna, S., (2019). Successful cloning of a superior buffalo Scientific Reports, 9(1), pp.1-9.
• Yadav, Satendra Kumar, Pawan Singh, Pradeep Kumar (corresponding author), S. V. Singh, Ajeet Singh, and Sunil Kumar (2019). Scrotal infrared thermography and testicular biometry: Indicator of semen quality in Murrah buffalo bulls. Animal Reproduction Science209: 106145.
• Saini, M., Selokar, N. L., Rajendran, R., Kumar, D., Kumar, P., & Yadav, P. S. (2019). Isolation and culture of epithelial cells from stored buffalo semen and their use for the production of cloned embryos. Reproduction, Fertility and Development, 31(10), 1581-1588.
• Kumar, P., Pawaria, S., Dalal, J., Ravesh, S., Bharadwaj, S., Jerome, A., … & Yadav, P. S. (2019). Sodium alginate potentiates antioxidants, cryoprotection and antibacterial activities of egg yolk extender during semen cryopreservation in buffalo. Animal Reproduction Science, 209,
• Kumar, P., Pawaria, S., Dalal, J., Bhardwaj, S., Patil, S., Jerome, A., & Sharma, R. K. (2019). Serum and seminal plasma IGF-1 associations with semen variables and effect of IGF-1 supplementation on semen freezing capacity in buffalo Animal Reproduction Science, 204, 101-110.
• Vijayalakshmy, K., Kumar, P., Virmani, M., Pawaria, S., Lalaji, N. S., Sharma, P., … & Kumar, D. (2018). A novel combination of silane‐coated silica colloid with hybrid RNA extraction protocol and    RNA    enrichment    for    downstream    applications    of     spermatozoal RNA. Andrologia, 50(6), e13030.
• Kumar, D., Sharma, P., Vijayalakshmy, K., Selokar, N. L., Kumar, P ., Rajendran, R., & Yadav, P. S. (2018). Generation of Venus fluorochrome expressing transgenic handmade cloned buffalo embryos using Sleeping Beauty transposon. Tissue and Cell, 51, 49-55.
• Kumar, , Saini, M., Kumar, D., Bharadwaj, A., & Yadav, P. S. (2017). Estimation of endogenous levels of osteopontin, total antioxidant capacity and malondialdehyde in seminal plasma: application for fertility assessment in buffalo (Bubalus bubalis) bulls. Reproduction in Domestic Animals, 52(2), 221-226.
• Swami, S., Kumar, Pradeep, Malik, R. K., Saini, M., Kumar, D., & Jan, M. H. (2017). The cryoprotective effect of iodixanol in buffalo semen cryopreservation. Animal Reproduction Science. 179: 20–26.
• Swami, S., Kumar, Pradeep, Malik, R. K., Saini, M., Kumar, D., & Jan, M. H. (2017). Cysteamine supplementation revealed detrimental effect on cryosurvival of buffalo sperm based on computer-assisted semen analysis and oxidative parameters. Animal Reproduction Science.177: 56-64.
• Kumar, Pradeep., Saini, , Kumar, D., Jan, M.H., Swami, D.S, Sharma, R.K. (2016). Quantification of leptin in seminal plasma of buffalo bulls and its correlation with antioxidant status, conventional and computer-assisted sperm analysis (CASA) semen variables. Animal Reproduction Science 166, 122-127.
• Kumar D, Kumar Pradeep, Singh P, Yadav SP and Yadav PS (2016). Assessment of sperm acrosome, plasma membrane, mitochondrial potential and DNA integrity by fluorescent probes in fresh, equilibrated and frozen-thawed buffalo Cytotechnology. 68: 451-458.
• Kumar, Pradeep., Saini, , Kumar, D., Bharadwaj A., Yadav PS (2016). Estimation of endogenous levels of osteopontin, total antioxidant capacity and malondialdehyde in seminal plasma: Application for fertility assessment in buffalo (Bubalus bubalis) bulls. Reproduction in Domestic Animals. 1-6. .
• Kumar Pradeep, Saini Monika, Kumar Dharmendra, Balhara A K, Yadav S P, Singh P and Yadav P S (2015). Liposome-based semen extender is suitable alternative to egg yolk-based extender for cryopreservation of buffalo (Bubalus bubalis) Animal Reproduction Science. 159: 38-45,
• Kumar Pradeep, Kumar Dharmendra, Sikka P and Singh P (2015). Sericin supplementation improves semen freezability of buffalo bulls by minimizing oxidative stress during Animal Reproduction Science. 152: 26–31