Assisted Reproductive Biotechnology including IVF, Stem cells, iPS cells, Cloning and Semen Biology

• 2^nd Best Poster Award received in National Conference of ISBD-2023, at DUVASU, Mathura, UP.
• Best Scientist Award of the 2022-23, ICAR-CIRB, Hisar
• Young Scientist Award received in XXXVII Annual Convention of ISSAR-2022, NDVASU, Jabalpur
• Best Second Paper Award received from XIX SOCDAB National Symposium- 2022, at ICAR-NBAGR, Karnal
• Limca Book of Records’ for ‘Most calves cloned from a single bull 2022
• Team Award received from ISBD-2021, at GADVASU, Ludhiana
• India Book of Record for ‘Maximum Buffalo Clones Produced-2021
• Associate Fellow 2021, National Academy of Dairy Science, India
• Nanaji Deshmukh ICAR Award for Outstanding Interdisciplinary Team Research 2019 for Animal Sciences & Fisheries during 92th Foundation Day of ICAR, New Delhi
• APA Mid-Career Award 2019, Animal Physiologists Association during APACON 2019 at Tirupati
• Dr S K Sirohi Young Researcher Award 2019 during 17th Convocation of ICAR-NDRI, Karnal

Top Ten Recent Research Publications (Last five years)

• Punetha M, Saini Sl, Choudhary S, Sharma S, Bala R, Kumar P, Sharma RK, Yadav PS, Datta TK, Kumar D. (2024). Establishment of CRISPR-Cas9 ribonucleoprotein mediated MSTN gene edited pregnancy in buffalo: Compare cells transfection and zygotes electroporation. Theriogenology 229:158–168. https://doi.org/10.1016/j.theriogenology.2024.08.027
• Jinagal S, Dutt R, Sharma M, Punetha M, Saini S, Thakur S, Chaudhary S, Kumar P, Yadav PS, Datta TK, Kumar D. LPS-Induced Mitochondrial Dysfunction Reduces Oocyte Maturation and Developmental Competence of Buffalo Embryos via ROS Mediated TLR4 Signalling. Am J Reprod Immunol. 2024; 92(1):e13902. doi: 10.1111/aji.13902.
• Kumari N, Saini S, Thakur S, Sharma S, Punetha M, Kumar P, Sango C, Sharma RK, Datta TK, Yadav PS, Kumar D. Enhancing the quality of inferior oocytes of buffalo for in vitro embryo production: The impact of melatonin on maturation, SCNT, and epigenetic modifications. Tissue Cell. 2024;89:102480. doi: 10.1016/j.tice.2024.102480.
• Sharma M, Punetha M, Saini S, Chaudhary S, Jinagal S, Thakur S, Kumar P, Kumar R, Sharma RK, Yadav PS, Kumar D. Mito-Q supplementation of in vitro maturation or in vitro culture medium improves maturation of buffalo oocytes and developmental competence of cloned embryos by reducing ROS production. Anim Reprod Sci. 2024;260:107382. doi: 10.1016/j.anireprosci.2023.107382.
• Punetha M, Kumar D, Saini S, Chaudhary S, Bajwa KK, Sharma S, Mangal M, Yadav PS, Green JA, Whitworth K, et al. Optimising electroporation condition for CRISPR/Cas-mediated knockout in zona-intact buffalo zygotes. Animals. 2024; 14(1):134. https://doi.org/10.3390/ani14010134
• Yadav PS, Kumar D, Saini M, Sharma RK, Dua S, Selokar NL, Bansal S, Punetha M, Gupta A, Kumar R, Kumar P. Evaluation of postnatal growth, hematology, telomere length and semen attributes of multiple clones and re-clone of superior buffalo breeding bulls. Theriogenology. 2024 Jan 1;213:24-33. doi: 10.1016/j.theriogenology.2023.09.024.
• Bajwa KK, Punetha M, Kumar D, Yadav PS, Long CR, Selokar NL. Electroporation-based CRISPR gene editing in adult buffalo fibroblast cells. Anim Biotechnol. 2023 Oct 23:1-12. doi: 10.1080/10495398.2023.2271030.
• Dua S, Bansal S, Gautam D, Jose B, Singh P, Singh MK, De S, Kumar D, Yadav PS, Kues W, Selokar NL. Production of MSTN gene-edited embryos of buffalo using the CRISPR/Cas9 system and SCNT. Cell Reprogram. 2023 Jun;25(3):121-127. doi: 10.1089/cell.2023.0003.
• Punetha M, Saini S, Chaudhary S, Bala R, Sharma M, Kumar P, Kumar D, Yadav PS. Mitochondria-targeted antioxidant MitoQ ameliorates ROS production and improves cell viability in cryopreserved buffalo fibroblasts. Tissue Cell. 2023 Jun;82:102067. doi: 10.1016/j.tice.2023.102067.
• Kues WA, Kumar D. Cocktails of defined chemical compound: sufficient to induce totipotency in embryonic stem cells. Signal Transduct Target Ther. 2022;7(1):330. doi: 10.1038/s41392-022-01184-8.