Major research interest focuses on the study of ovarian physiology in relation to fertility and infertility in buffalo and its manipulation for optimisation of reproductive efficiency of the species. Current research efforts include study of ovarian follicular dynamics under physiological / pathological conditions and hormonal interventions together with study of genomic and physiological regulation of folliculogenesis and early embryonic development in buffalo. Other research aspects being addressed are semen biology, embryo biotechnology, stem cell technology and stress physiology.

• Shakya J, Balhara AK, Dahiya SS, Lailer PC and Singh I (2019) Improved dairy production through enzyme supplementations. Indian Journal of Animal Science (accepted)
• Kavya KM, Sharma RK, Jerome A, Phulia SK, Balhara AK and Singh I (2018) Blood metabolites, body condition score, body weight and milk yield in relation to resumption of cyclicity in post-partum buffaloes. Indian Journal of Animal Sciences 88 (10): 1142–1145
• Verma R, Singh I, Balhara AK, Nayan V, Sharma RK and Chaudhary V (2018). Correlation between extremes age at first calving with their productive and reproductive performances in Indian Murrah buffaloes (Bubalus bubalis). Indian Journal of Animal Research 52(10): 1506-1512
• Balhara AK, Nayan V, Dey A, Singh KP, Dahiya SS and Singh I (2017) Climate change and buffalo farming in major milk producing states of India – present status and need for addressing concerns. Indian Journal of Animal Sciences 87 (4): 403–411
• Buragohain L, Nanda T, Ghosh A, Ghosh M, Kumar R, Kumar S, Gupta SS, Bharali A, Mohanty AK, Singh I and Balhara AK (2017) Identification of serum protein markers for early diagnosis of pregnancy in buffalo. Animal Science Journal. 88(8):1189-1197
• Kumar R., Ghosh M., Kumar N., Balhara AK, Gupta M, Sharma RK and Singh I (2017) Polymorphism in 5′ untranslated region of Heat Shock Protein 70 gene as marker of post-partum anestrus in Murrah buffaloes. Reproduction in Domestic Animals. 52:505–512
• Buragohain L, Nanda T, Ghosh A, Ghosh M, Kumar R, Kumar S, Gupta SS, Bharali A, Mohanty AK, Singh I and Balhara AK (2016) Identification of serum protein markers for early diagnosis of pregnancy in buffalo. Animal Science Journal 88(8):1189-1197
• Singh I and Balhara AK (2016) New approaches in buffalo artificial insemination programs with special reference to India. Theriogenology 86(1):194-199
• Ramadan TA, Sharma RK, Phulia SK, Balhara AK, Ghuman SS, Singh I (2016) Manipulation of reproductive performance of lactating buffaloes using melatonin and controlled internal drug release device treatment during out-of-breeding season under tropical conditions. Theriogenology 86(4):1048-1053
• Buragohain L, Kumar R, Nanda T, Phulia SK, Mohanty AK, Kumar S, Sunesh, Ghuman SPS, Singh I and Balhara AK (2016) Serum MX2 protein as candidate biomarker for early pregnancy diagnosis in buffalo. Reproduction in Domestic Animals 51(4):453-460
• Rawat P, Bathla S, Baithalu R, Yadav ML, Kumar S, Ali SA, Tiwari A, Lotfan M, Naru J, Jena M, Behere P, Balhara AK, Vashisth R, Singh I, Dang A, Kaushik JK, Mohanty TK and Mohanty AK (2016) Identification of Potential Protein Biomarkers for Early Detection of Pregnancy in Cow Urine using 2D DIGE and Label Free Quantitation. Clinical Proteomics13:15. doi: 10.1186/s12014-016-9116-y
• Bathla S, Rawat P, Baithalu R, Yadav ML, Naru J, Tiwari A, Kumar S, Balhara AK, Singh S, Chaudhary S, Kumar R, Lotfan M, Behare P, Phulia SK, Mohanty TK, Kaushik JK, Nallapeta S, Singh I, Ambatipudi SK and Mohanty AK (2015) Profiling of urinary proteins in Karan Fries cows reveals more than 1550 proteins. Journal of Proteomics 127: 193–201
• Balhara AK, Gupta M, Mohanty AK, Phulia SK, Sharma RK, Singh S and Singh I (2014) Changes in sera proteome in relation to day of pregnancy in early pregnant buffaloes. Indian Journal of Animal Sciences 84 (4): 400–409
• Balhara AK , Mohanty AK , Gupta M , Jamwal M, Sunesh, Phulia SK and Singh I (2014) Bioinformatics and functional analysis of proteins in serum of early pregnant buffaloes. Indian Journal of Animal Sciences 84 (2): 140–145
• Ghosh M, Gupta M, Kumar R, Kumar S, Balhara AK, Singh I (2015) Relation between antioxidant status and postpartum anestrous condition in Murrah buffalo, Veterinary World 8(10): 1163-1166
• Kumar S, Balhara AK, Kumar R, Kumar N, Buragohain L, Baro D, Sharma RK, Phulia SK, Singh I (2015) Hemato-biochemical and hormonal profiles in post-partum water buffaloes (Bubalus bubalis), Veterinary World 8(4); 512-517
• Sadeesh EM, Shah F, Balhara AK, Thirumaran SMK, Balhara S, Kumar D, Yadav S, Singh I, Yadav PS (2014). Effect of hormones, sera, follicular fluid and oocyte quality on in vitro nuclear maturation and fertilization rate of Indian water buffalo (Bubalus bubalis) oocytes. Journal of Cell and Tissue Research 14 (1): 4165– 4171.
• Kumar R, Gupta M, Shafiq S, Balhara AK, Sadeesh EM, Ghosh M and Singh I (2014). Sequencing–based polymorphism in leptin receptor gene and lack of association with postpartum anestrus in Murrah buffaloes. Turkish Journal of Veterinary and Animal Sciences 38 (6): 670–674.
• Kumar R, Gupta M, Shafiq S, Balhara AK, Sadeesh EM and Singh I (2014). Leutinising hormone receptor gene polymorphism and its association with post partum anestrus in Murrah buffaloes. Journal of Cell and Tissue Research 14 (2): 4237– 4240.
• Ramadan TA, Sharma RK, Phulia SK, Balhara AK, Ghuman SS, Singh I (2014) Effectiveness of melatonin and controlled internal drug release device treatment on reproductive performance of buffalo heifers during out-of-breeding season under tropical conditions. Theriogenology 82:1296–1302
• Balhara AK , Mohanty AK , Gupta M , Jamwal M, Sunesh, Phulia SK and Singh I (2014) Bioinformatics and functional analysis of proteins in serum of early pregnant buffaloes. Indian Journal of Animal Sciences 84 (2): 140–145
• Balhara AK, Gupta M, Mohanty AK, Phulia SK, Sharma RK, Singh S and Singh I (2014) Changes in sera proteome in relation to day of pregnancy in early pregnant buffaloes. Indian Journal of Animal Sciences 84 (4): 400–409
• Balhara AK, Gupta M, Singh S, Mohanty AK and Singh I (2013) Early Pregnancy Diagnosis in Bovines: Current Status and Future Directions. The Scientific World Journal. Published online Article ID 958540, 10 pages, doi:10.1155/2013/958540
• Balhara AK, Phulia SK, Nayan V and Singh I (2012) Minimally invasive muscle biopsy technique for molecular studies in buffaloes. Indian Veterinary Journal 89(9): 119-121